Regulatory
Part:BBa_K1343017:Design
Designed by: Tal Naor, Ricarina Rabinovitz, Ittai Rubinstein, Yair Maimon Group: iGEM14_Technion-Israel (2014-10-06)
Ptet->tetR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 63
Illegal XhoI site found at 56 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
This promoter is induced by aTc. The gene tetR, results in the formation of the repressor tetR. TetR represses the promoter Ptet. It can be used as a tuner, to repress itself as a function of the aTc concentration present. It allows us to control the functionality of the system it is incorparated in.
Source
BBa_R0040:TetR repressible promoter, BBa_B0030: Strong RBS, BBa_B0010:T1 (terminator) from E. coli.