Regulatory

Part:BBa_K1343017:Design

Designed by: Tal Naor, Ricarina Rabinovitz, Ittai Rubinstein, Yair Maimon   Group: iGEM14_Technion-Israel   (2014-10-06)

Ptet->tetR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 63
    Illegal XhoI site found at 56
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


This promoter is induced by aTc. The gene tetR, results in the formation of the repressor tetR. TetR represses the promoter Ptet. It can be used as a tuner, to repress itself as a function of the aTc concentration present. It allows us to control the functionality of the system it is incorparated in.

Source

BBa_R0040:TetR repressible promoter, BBa_B0030: Strong RBS, BBa_B0010:T1 (terminator) from E. coli.

References